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KMID : 0861020220370050001
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Korea Journal of Herbology
2022 Volume.37 No. 5 p.1 ~ p.8
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Effects of a Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos Extract on Estrogenic Activities and Anti-Osteoclastogenesis
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Park Dong-Jun
Lee Hong-Gu
Park Hyoung-Kook
Jin Mu-Hyun
Cho Ho-Song
Min Kyoung-In
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Abstract
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Objectives : This study aimed to investigate the synergistic effect of combining Cynanchi Wilfordii Radix extract with
Humuli Lupuli Flos extract on estrogenic and anti-osteoclastogenic activity.
Methods : Estrogenic effect of a mixture of Cynanchi Wilfordii Radix extract and Humuli Lupuli Flos extract (CWHL), Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin (an active ingredient of Cynanchi wilfordii
Radix extract) and 8-prenylnaringenin (an active ingredient of Humuli Lupuli Flos extract) were examined by
proliferation E-screen assay and expression of estrogen inducible gene, pS2 via Real Time-PCR (RT-PCR) in MCF-7
estrogen responsive cells. And their estrogenic activities were investigated how to modulate Estrogen receptor β by
binding affinity assay. Inhibitory effect of CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract,
caudatin and 8-prenylnaringenin on RANKL-induced osteoclast differentiation were tested by TRAP (Tartrate-resistant
acid phosphatase) staining in osteoclastogenic RAW 264.7 cells.
Results : CWHL, Humuli Lupuli Flos extract and 8-prenylnaringenin accelerated the proliferation of MCF-7 and the
expression of pS2 in MCF-7. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and
8-prenylnaringenin bind to estrogen receptor β. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract,
caudatin and 8-prenylnaringenin inhibited RANKL-induced osteoclastogenesis in osteoclastogenic RAW 264.7. CWHL
is more effective for all markers than Cynanchi Wilfordii Radix extract or Humuli Lupuli Flos extract alone.
Conclusions : CWHL may a potential therapeutic agent for menopause and osteoporosis as a natural food resource.
CWHL as a natural food source has therapeutic potential in cases of menopause and osteoporosis. 1)
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KEYWORD
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CWHL, Cynanchi Wilfordii Radix, Humuli Lupuli Flos, Estrogenic Activities, Anti-osteoclastogenesis
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